New GPCRs from a Human Lingual cDNA Library
نویسندگان
چکیده
منابع مشابه
Transcripts from a human primordial follicle cDNA library.
BACKGROUND Human primordial follicles (PFs) or the oocyte-pre-granulosa complex, constitute the earliest and most immature stage of human oogenesis. The factors, signalling networks and the precise role of the oocyte and the pre-granulosa cells in initiating growth and recruitment from this finite resting pool remain largely unknown at present. METHODS To obtain a gene resource of this oogene...
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Mammalian Gene Collection (MGC) verified over 9,000 human full-ORF genes and FLJ Program reported 21,243 cDNAs of which 14,409 were unique ones and 5,416 seemed to be protein-coding. The pity is that epididymis cDNA library was missing in their sequencing target list. Epididymis is a very important male accessory sex organ for sperm maturation and storage. Fully differentiated spermatozoa left ...
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Human fetal muscle cDNA library was screened with a beta-myosin heavy chain gene fragment containing Alu sequences. Two cDNA clones AI and BII with 1.8 and 3 kb inserts respectively were chosen for further characterization by means of RNA and DNA hybridization procedures and sequencing. The clones appeared to contain repetitive sequences as well as single copy regions. They are actively transcr...
متن کاملIdentification of genes expressed in a human scleral cDNA library.
PURPOSE Clones established from a human scleral cDNA library were systematically sequenced. Public database sequence comparisons were performed to generate a profile of genes expressed in the human sclera and identify candidate genes for inherited diseases with scleral involvement. METHODS A directionally cloned pCMV-PCR cDNA library was constructed from RNA isolated from scleras of human don...
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We report the construction of a plasmid-based cDNA library made from microdissected cells derived from prostatic intraepithelial neoplasia. Total RNA was extracted and converted to blunt-ended, double-stranded cDNA by oligo(dT)-mediated reverse transcription followed by linker addition. A linker-specific primer with UDG-compatible ends was used to amplify the cDNA and the resulting PCR product ...
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ژورنال
عنوان ژورنال: Chemical Senses
سال: 2001
ISSN: 1464-3553
DOI: 10.1093/chemse/26.9.1157